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TEST ID UCULT CULTURE, URINE

Important Note

Intended for the quantitation and identification of urinary pathogens

Susceptibility testing will only be performed when applicable

 

**Collection method is required**

Infectious

Performing Laboratory

NRLS-Microbiology

Specimen Type

Urine in sterile container 

Urine in boric acid preservative

 

Acceptable urine sources include: 

  Urine Clean Catch

  Urine, Catheterized (Straight, <7 day foley, or >7 day foley)

  Urine, Kidney  

  Urine, Suprapubic

                                                        

Specimen Required

Clean-voided, midstream urine  (Urine, Clean catch):

    Females:

1.  Wash your hands with soap and water before beginning collection.

2.  Clean genital area by spreading the labia and washing the vulva with supplied cleansing wipes from front to back.

3.  Keep the labia separated and urine a small amount of urine into the toilet, then use the sterile cup the collect the "midstream" urine.

4.  Finish urinating into the toilet.

5.  Replace cap on the cup and secure.

    Males:

1.  Wash your hands with soap and water before collection.

2.  Using the cleansing wipes provided, cleanse the head of the penis in a circular motion with the first towelette.  If patient is uncircumcised, retract the foreskin before cleansing.

3.  Repeat this step with the second cleansing wipe.

4.  Keeping the foreskin retracted, urinate a small amount of urine into the toilet, then using the cup collected the "midstream" urine.

5.  Finish urinating into the toilet

6.  Replace the cap and secure tightly

Catheterized Urine:

  Straight cath:  Performed by physician or trained health professional

  -Use aesptic technique.  Discard the first 15-30 ml of urine, then collect next flow of urine for culture

   Foley Catheter:

  -Using a needle and syringe, collec the urine through the catheter port after cleaning with alcohol.  

  -Alternatively, collect the sample directly into a vacutainer tube (without additives) using a vacutainer holder and needle.

***DO NOT SEND URINE OBTAINED FROM THE CATHETER BAG**

Suprapubic:

  -To be performed by physician or trained medical professional

  -Bladder should be full and palpable before aspiration

  -Save and disinfec the skin over the bladder

  -Make a small lancet wound through the epidermis and above the pubis symphysis

  -Aspirate using a needle and syringe.  Dispense the collected urine into a sterile cup 

Ileal conduit or Urostomy:

  -Remove the external device

  -Cleanse the stoma with 70% alcohol, followed by iodine, then followed by alcohol.

  -Insert a double catheter into the cleansed stoma to a depth beyond the fascial level and collect the urine.

Pediatric "Pedi-bag" or "U-bag" :

  -Submit in sterile container within 2 hours of collection.

  

-Label cup with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, urine source, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature.

 

 

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine-Boric Acid preservative (grey top) Room Temperature 72 hours
  Refrigerated 72 hours
Urine-No preservatives Refrigerated 24 hours
  Room Temperature 2 hours

Reference Values

An interpretive report will be provided

Day(s) Performed

Sunday through Saturday

Report Available

Preliminary report available within 24 hours.  Final report available within 24-48 hours

 

Rejection Criteria

-Collection from a bag of a catheterized urine

-Specimen exceeds stability requirements

-Duplicate specimens (same collection method) submitted within 24 hours of receipt of the first

-Anaerobic bacteriology is by special request only and will be performed from rapidly transported suprapubic aspirates or unusual medical conditions

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