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TEST ID VRE CULTURE, VANCOMYCIN RESISTANT ENTEROCOCCUS (VRE) SCREEN

Important Note

Intended as a method of screening for fecal colonization of vancomycin-resistant enterococcus

 

**NOTE:  The presence of VRE is an indication of carriage and/or colonization.  It does not indicate infection since enterococci is considered normal fecal flora

Infectious

Performing Laboratory

NRLS-Microbiology

Specimen Type

RECTAL SWAB -(BD Eswab transport system OR other culture collection swabs (Red top))

STOOL SAMPLE- Raw specimen in sterile container, Cary Blair stool transport, or FecalSwab transport

Specimen Required

RECTAL SWAB:

1.  Insert the swab 1-2 inches into the anal canal and collect feces.  Fecal soiling needs to be present on the swab.

-Label swab with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature.

STOOL SAMPLE:

Pass stool into a clean, dry container. 

--The use of a bed pan or a large plastic bag into a waste basket can be used to catch the specimen----Label cup with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature.

For Cary Blair media (Orange top transport):

1)  Open the lid of the tube containing the liquid. 

2)  Using the built-in spoon, place small spoonfuls of stool (sample the bloody, slimy, or watery portions) into the tube until the liquid level rises to the red line.   

3)  If the stool is formed, try to sample small amounts from each end/side and the middle. 

4)  Mix the contents with the spoon, then twist the cap tightly closed and shake to mix well.

For the FecalSwab transport:

1) Remove the swab from the package, making sure to only hand the swab by the shaft

2)  Collect the stool onto the swab by inserting the swab tip into the stool sample and rotate.  Be sure to sample the blood, slimy, or watery areas.  DO NOT try to collect a large amount of sample on the swab, but fecal material must be visible on the swab.

3)  Carefully insert the swab into the vial of fluid that came with the swab (orange top tube) and mix the specimen into the fluid by swirling against the inside of the tube.

4)  Break off the swab at the perforation into the tube by holding the top of the swab shaft and bending toward you.  Discard the top portion of the swab and replace the orange lid.

5)  Shake well until mixed.  Confirm that the liquid level is not above the noted line on the tube.

 

-Label transport container with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature

 

Specimen Minimum Volume

One rectal swab

     or

One stool sample (Raw sample, Cary Blair, or Fecal Swab)

Specimen Stability Information

Specimen Type Temperature Time
Rectal swab (Red top) Room Temperature 24 hours
Rectal swab (BD Eswab) Room Temperature 48 hours
Raw Stool Refrigerated 2 hours
Cary Blair or FecalSwab Room Temperature 72 hours

 

Reference Values

An interpretive report will be provided

Day(s) Performed

Sunday through Saturday

Report Available

Preliminary report available within 24 hours.  Final report available at 48 hours.