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HelpTEST ID VITB3 Vitamin B3 and Metabolites, Plasma
Specimen Required
Patient Preparation:
Fasting: 4 hours, preferred but not required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Lavender top (K2 EDTA)
Acceptable: K3 EDTA
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL Plasma
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot plasma into a plastic vial.
Useful For
Assessment of vitamin B3 status
Aid in the diagnosis of pellagra
Assisting in the diagnosis of suspected vitamin B3 deficiency or toxicity
Monitoring of vitamin B3 therapy
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin B3 and Metabolites, PSpecimen Type
Plasma EDTASpecimen Minimum Volume
Plasma: 0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 21 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
Nicotinic Acid (Niacin) Cutoff: <5.0 ng/mL
Nicotinamide: 5.0-48.0 ng/mL
Nicotinuric Acid Cutoff: <5.0 ng/mL
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Report Available
2 to 4 daysSpecimen Retention Time
2 weeksPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84591