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TEST ID TRICHPCR TRICHOMONAS VAGINALIS BY NUCLEIC ACID AMPLIFICATION

Important Note

In vitro qualitative nucleic acid amplification test for the detection of ribosomal RNA from Trichomonas vaginalis

 

**Swab specimen transport tubes with no swabs, two swabs, a cleaning swab, or a swab not provided in the APTIMA collection kit will be rejected**

**If test request is for urine, please refer to URTRICHPCR**

 

Infectious

Additional Codes

 

 

Performing Laboratory

NRLS-Microbiology

Specimen Type

Unisex APTIMA swab (endocervical)

Multitest APTIMA swab (vaginal)

PreservCyt solution liquid pap specimen (ThinPrep)

 

Specimen Minimum Volume

One swab (blue or pink) in APTIMA transport containing 2.9ml of transport medium

1 ml PreservCyt solution in ThinPrep container 

Specimen Stability Information

Specimen Type Temperature Time
APTIMA Unisex and/or Multitest swab Refrigerated or Ambient 60 days
PreservCyt solution (ThinPrep vial) Refrigerated or ambient 30 days

 

Reference Values

An interpretive report will be provided

Specimen Collection

Unisex APTIMA swabs

  • Endocervical swab collection:
    • Remove excess mucus from cervical os and surrounding mucosa using the cleaning swab (white swab).  Discard this swab.
    • Insert specimen collection swab (blue swab) into the endocervical canal
    • Gently rotate swab clockwise for 10-30 seconds in the endocervical canal
    • Withdraw the swab carefully, avoiding the vaginal mucosa
    • Remove foil cap from specimen transport tube and immeidately place collection swab into the transport tube with solution
    • Break off the swab at the scoreline and recap

Multitest APTIMA swab 

  • Vaginal swab specimen collection 
    • Peel open the swab package and remove the swab.  
    • Hold the swab, placing your thumb and forefinger in the middle of the swab shaft covering the score line.  
    • Carefully insert the swab into the vagina about 2 inches, past the introitus and gently rotate for 10-30 seconds.  Make sure the swab touches the walls of the vagina so that the moisture is absorbed by the swab.
    • Remove the swab without touching the skin
    • Remove the foil cap from the Multitest tube and immediately place the swab into the transport media.
    • Carefully break the swab shaft at the score line against the side of the tube and discard the top portion of the swab
    • Replace the foil cap and transport to the laboratory

PreservCyt solution vials (ThinPrep Pap test)

  • Please refer to ThinPrep Pap Test collection procedures
    • After transport of the liquid pap to the laboratory, the laboratory will transfer 1ml of specimen to the APTIMA specimen transfer tube for processing.

 

Methodology

Transcription Mediated Amplification (TMA)