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HelpTEST ID TDP Thiamine (Vitamin B1), Whole Blood
Reporting Name
Thiamin (Vitamin B1), WBSpecimen Type
Whole Blood EDTAShipping Instructions
Ship specimen in amber vial or tube to protect from light.
Specimen Required
Patient Preparation:
1. Patient should fast overnight (12-14 hours); infants should have specimen collected just before next feeding. Water can be taken as needed.
2. For 12 hours before specimen collection, patient should not take vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Amber vial
Specimen Volume: 4 mL
Collection Instructions:
1. Invert 8 to 10 times to mix blood.
2. Transfer whole blood into amber vial or tube and freeze within 24 hours of collection.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Frozen | 28 days | LIGHT PROTECTED |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reject Due To
| Gross lipemia | Reject |
| Glass vial Clotted specimen |
Reject |
Reference Values
70-180 nmol/L
Day(s) Performed
Monday through Friday
Report Available
3 to 6 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84425
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Useful For
Assessment of thiamine deficiency
Measuring thiamine levels in patients with behavioral changes, eye signs, gait disturbances, delirium, and encephalopathy; or in patients with questionable nutritional status, especially those who appear at risk and who also are being given insulin for hyperglycemia
Highlights
-Whole blood thiamine testing is superior to currently available alternative tests for assessing thiamine status. Serum or plasma thiamine testing suffers from poor sensitivity and specificity, and less than 10% of blood thiamine is contained in plasma.
-Thiamine diphosphate (TDP) is the active form of thiamine and is most appropriately measured to assess thiamine status. Thiamine diphosphate in circulating blood is present in erythrocytes but is undetectable (present in very low levels) in plasma or serum.
-Liquid chromatography tandem mass spectrometry analysis of TDP in whole blood is the most sensitive, specific, and precise method for determining the nutritional status of thiamine and is a reliable indicator of total body stores.
-This assay specifically targets and quantitates the active form of thiamine, TDP, as an indicator of thiamine status.