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TEST ID TCD4 CD4 Count for Immune Monitoring, Blood

Additional Codes

Aliases

T-Helper/T-Suppressor-Flow Cytometry

T4/T8 Helper Suppressor Ratio

CD4 Count, CD8 Count, Flow Cytometry, Helper Suppressor Ratio, Immune Competence

Immune Status-Flow Cytometry, Immunodeficiency Panel-Flow Cytometry

Quantitative CD4 and CD8, T Cell, T Cells, T Lymphocyte Surface Markers

T-Cell Surface Markers, T-Cells

Reporting Name

CD4 T-Cell Count

Specimen Type

Whole Blood EDTA


Ordering Guidance


For diagnosing T-lymphocytic malignancies or evaluation of T-cell lymphocytosis of unknown etiology, order LCMS / Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Varies, which includes a hematopathology review.



Shipping Instructions


It is recommended that specimens arrive within 24 hours of collection. Collect and package specimen as close to shipping time as possible.



Necessary Information


Date of collection is required.



Specimen Required


Container/Tube: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.

Additional Information: For serial monitoring, it is recommended that specimen collection be performed at the same time of day.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Ambient 72 hours PURPLE OR PINK TOP/EDTA

Method Name

Flow Cytometry

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject

Reference Values

The appropriate age-related reference values will be provided on the report.

Day(s) Performed

Monday through Sunday

Report Available

1 to 2 days

Specimen Retention Time

4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

86359-T cells, total count

86360-Absolute CD4/CD8 count with ratio

Useful For

Serial monitoring of CD4 T cell count in patients who are HIV-positive

 

Follow-up and diagnostic evaluation of primary cellular immunodeficiencies, including severe combined immunodeficiency

 

T-cell immune monitoring following immunosuppressive therapy for transplantation, autoimmunity, and other immunological conditions where such treatment is utilized

 

Assessment of T-cell immune reconstitution post hematopoietic cell transplantation

 

Early screening of gross quantitative anomalies in T cells in infection or malignancies

 

This assay should not be used for diagnosing T-lymphocytic malignancies or evaluation of T-cell lymphocytosis of unknown etiology.