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TEST ID PREGN Pregnenolone, Serum

Reporting Name

Pregnenolone, S

Specimen Type

Serum


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge the specimen and immediately aliquot serum into a plastic vial.

2. Freeze immediately.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen 28 days

Special Instructions

Testing Algorithm

For more information see Steroid Pathways.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Reference Values

CHILDREN/ADOLESCENTS*

Males

0-6 years: Not established

7-9 years: <206 ng/dL

10-12 years: <152 ng/dL

13-15 years: 18-197 ng/dL

16-17 years: 17-228 ng/dL

Tanner Stages

Stage I: <157 ng/dL

Stage II: <144 ng/dL

Stage III: <215 ng/dL

Stage IV-V: 19-201 ng/dL

 

Females

0-6 years: Not established

7-9 years: <151 ng/dL

10-12 years: 19-220 ng/dL

13-15 years: 22-210 ng/dL

16-17 years: 22-229 ng/dL

Tanner Stages

Stage I: <172 ng/dL

Stage II: 22-229 ng/dL

Stage III: 34-215 ng/dL

Stage IV-V: 26-235 ng/dL

 

*Kushnir MM, Rockwood AL, Roberts WL, et al. Development and performance evaluation of a tandem mass spectrometry assay for 4 adrenal steroids. Clin Chem. 2006;52(8):1559-1567

 

ADULTS

≥18 years: 33-248 ng/dL

 

To convert to nmol/L, multiply the value in ng/dL by 0.03159757.

Day(s) Performed

Monday through Wednesday, Friday

Report Available

3 to 7 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

84140

Useful For

An ancillary test for congenital adrenal hyperplasia, particularly in situations in which a diagnosis of both 21- and 11-hydroxylase deficiency have been ruled out

 

Confirming a diagnosis of 3-beta-hydroxy dehydrogenase deficiency