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TEST ID LENT Enterovirus, Molecular Detection, PCR, Varies

Reporting Name

Enterovirus PCR

Specimen Type

Varies


Ordering Guidance


This test will detect enterovirus but will not differentiate viruses in this family or provide serotyping information.



Necessary Information


1. Specimen source is required.

2. Source information should include main anatomical site of collection.



Specimen Required


Submit a raw clinical sample (not a culture isolate) for enterovirus testing.

 

Submit only 1 of the following specimens:

 

Specimen Type: Body fluid

Sources: Pericardial, peritoneal

Container/Tube: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Submit specimen from collection vial 2.

2. Do not centrifuge.

 

Specimen Type: Swab

Supplies: Culturette (BBL Culture Swab) (T092)

Sources: Dermal, eye, rectal, genital, nasopharyngeal, oropharyngeal, throat, nasal, or urethral

Container/Tube: Multimicrobe media (M4-RT) or similar viral transport media (M4 or M5) and Eswab

Specimen Volume: Entire specimen

Collection Instructions:

1. Rectal swab must have no visible fecal matter

2. Place swab back into multimicrobe media (M4-RT, M4, or M5)

 

Specimen Type: Respiratory

Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, pleural fluid, sputum, or tracheal aspirate

Container/Tube: Sterile container

Specimen Volume: 1.5 mL

Collection Instructions: Do not centrifuge.


Specimen Minimum Volume

Body and Respiratory fluids: 0.5 mL; Spinal fluid: 0.3 mL; Swab: See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm

Method Name

Real-Time Polymerase Chain Reaction (PCR)/RNA Probe Hybridization

Reject Due To

Calcium alginate-tipped swab
Wood swab
Transport swab containing gel
Heat-inactivated specimen		 Reject
 

Reference Values

Negative

Day(s) Performed

Monday through Sunday

Report Available

2 to 3 days

Specimen Retention Time

7 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87498

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Useful For

Aiding in diagnosing enterovirus infections

 

This test should not be used to screen asymptomatic patients.