SPECIMEN COLLECTION

Collection of the BASELINE breath sample:

1.  Remove the cap from the mouthpiece of the blue BASELINE bag.

2.  Instruct the patient to take a deep breath, hold their breath for 4 to 5 seconds and then exhale directly into the mouthpiece of the blue BASELINE breath bag until completely full.

3.  If the bag is not full repeat step II.

4.  Replace the cap on the bag mouthpiece and firmly press until it clicks and is securely locked into place.

Note: If the patient has not held their breath for 4-5 seconds or does not fill the bag completely, there is a possibility a test result will not be obtainable.

Note: The bag is not fully closed if the cap does not click into place. Not fully closing the bag my cause the breath sample to slowly leak out.

Prepare the test drink:

Prepare the test drink no more than 120 minutes before administering it to the patient. This is the maximal time for maintaining solution stability.

1.  Dissolve the Citrica powder and the C-enriched urea tablet in 5.1-6.8oz. of room temperature tap water in the provided drinking cup supplies with the kit.

2.  Close the provided lid, close the lid firmly using both hands.  Place fingers over the lid and shake thoroughly for a few minutes, until the powder and tablet are completely dissolved.

Note:  Tiny particles may remain visible after thoroughly mixing.  However, if more substantial particulate matter is still present after five minutes of stirring, discard the solution and repeat the procedure with a new kit.

Administering the test drink:

1.  Give the prepared test drink to the patient.

2.  Ensure that the patient drinks the solution through the straw.

3.  The patient, including pediatric patients aged 3-17 regardless of age and bodyweight, must drink the solution within two minutes and consume the entire amount.

4.  Start the timer for 15 minutes.

5.  After the patient finishes drinking the solution, record the present time plus (+) another 15 minutes in the Time to Fill field on the gray POST INGESTION bag.

Collection of the POST INGESTION breath sample:

1.  Fifteen minutes after the administration of the test drink (but not later than 20 minutes after administration) remove the cap from the mouthpiece of the gray POST INGESTION bag.

2.  Instruct the patient to take a deep breath, hold their breath for 4 to 5 seconds and then exhale directly into the mouthpiece of the gray POST INGESTION bag until it is full.

3.  If the bag is not full repeat step II.

4.  Replace the cap on the bag mouthpiece and firmly press until it clicks and is securely locked into place.

5.  Confirm the bags are properly labeled with patient name and identifiers. 

6.  Place both bags in the provided transport bag and transport to the laboratory.

Note: If the patient has not held their breath for 4-5 seconds or does not fill the bag completely, there is a possibility a test result will not be obtainable.

Note: The bag is not fully closed if the cap does not click into place. Not fully closing the bag my cause the breath sample to slowly leak out.