TEST ID HIVQN HIV-1 RNA Detection and Quantification, Plasma
Reporting Name
HIV-1 RNA Detect/Quant, PSpecimen Type
Plasma EDTAShipping Instructions
Ship specimen frozen on dry ice. If shipment will be delayed for more than 24 hours, freeze plasma specimen at -20 to -80° C until shipment and transport on dry ice.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions:
1. Centrifuge blood collection tube and aliquot plasma into a plastic vial per manufacturer's instructions (eg, centrifuge and aliquot within 2 hours of collection for BD Vacutainer tubes).
2. Freeze aliquoted plasma for shipment.
Specimen Minimum Volume
0.8 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Plasma EDTA | Frozen (preferred) | 84 days | |
Refrigerated | 6 days |
Special Instructions
Testing Algorithm
The following algorithms are available:
-HIV Prenatal Testing Algorithm, Including Follow-up of Reactive Rapid Serologic Test Results
Method Name
Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Reject Due To
Gross hemolysis | OK |
Gross lipemia | OK |
Gross icterus | OK |
Reference Values
Undetected
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysSpecimen Retention Time
60 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterCPT Code Information
87536
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-Microbiology Test Request (T244)
-General Request (T239)
Useful For
Quantifying plasma HIV-1 RNA levels (viral load) in individuals living with HIV-1:
-Before initiating antiretroviral therapy to obtain baseline viral load
-Who may have developed HIV-1 drug resistance while on antiretroviral therapy
-Who may be noncompliant with antiretroviral therapy
Monitoring HIV-1 disease progression before or during antiretroviral drug therapy