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TEST ID HCVG Hepatitis C Virus Genotype, Serum

Reporting Name

HCV Genotype, S

Specimen Type

Serum SST


Shipping Instructions


Ship specimen frozen on dry ice only. If shipment will be delayed for more than 3 days, freeze serum at -20° C or colder (up to 42 days) until shipment on dry ice.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Serum gel (red-top tubes are not acceptable)

Submission Container/Tube: Plastic vial

Specimen Volume: 5 mL

Collection Instructions:

Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.

Additional Information:

1. This test requires a minimum hepatitis C virus viral load of 500 IU/mL within the 30 days preceding collection.

2. Serum specimens previously submitted to other laboratories for non-microbiology tests are not acceptable for add-on test requests due to possible sample-to-sample carryover from automation used for those tests.


Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum SST Frozen (preferred) 42 days ALIQUOT TUBE
  Refrigerated  72 hours ALIQUOT TUBE

Testing Algorithm

Specimens either generating indeterminate genotype results, results with multiple or mixed hepatitis C virus genotypes (eg, 1, 5; 1, 2; or 1, 5), or those containing genotype 1 with no subtype will be automatically evaluated with by genotype resolution at an additional charge.

 

The following algorithms are available:

-Chronic Hepatitis C Treatment and Monitoring Algorithm: Direct Antiviral Antigen (DAA) Combination

-Hepatitis C: Testing Algorithm for Screening and Diagnosis

Method Name

Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) followed by Hybridization with Sequence-Specific, Fluorescent-Labeled Oligonucleotide Probes

Reject Due To

Gross hemolysis OK
Gross lipemia OK

Reference Values

Undetected

Day(s) Performed

Monday through Friday

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
HCVGR HCV Genotype Resolution, S No No

Report Available

1 to 6 days

Specimen Retention Time

2 months

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87902

Forms

If not ordering electronically, complete, print, and send 1 of the following:

-Gastroenterology and Hepatology Test Request (T728)

-Microbiology Test Request (T244)

Useful For

Determining hepatitis C virus (HCV) genotype (1 to 5) to guide antiviral therapy in patients with chronic hepatitis C

 

Differentiating between HCV subtypes 1a and 1b

 

This assay should not be used as a screening test for HCV infection. It should be performed only on specimens obtained from patients confirmed to have HCV RNA levels in serum of 500 IU/mL or higher.