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TEST ID GCCULT CULTURE, NEISSERIA GONORRHOEAE (GC)

Important Note

Intended for the isolation and identification of Neisseria gonorrhoeae from culture.

**Vaginal specimens are not optimal for the diagnosis of gonorrhea in women and should be reserved for only the evaluation of preteen-age girls**

**Due to the sensitive nature of this organism and recovery, it is recommended that the CHLAMYDIA TRACHOMATIS/NEISSERIA GONORRHOEAE BY NUCLEIC ACID AMPLIFICATION (CTGCPCR) be utilized for genital sources**

Infectious

Performing Laboratory

NRLS-Microbiology

Specimen Type

Sources include:  Cervix, Penis, Rectum, Throat, Urethra, and Vaginal

Preferred collection device:  BD Eswab or BD Eswab mini-tip

Specimen Required

ENDOCERVICAL/CERVIX:

   DO NOT USE LUBRICANT FOR THIS PROCEDURE

1.  Wipe the cervix clean of vaginal secretions and mucus

2.  Using the BD E-swab, obtain exudate from the endocervial glands.  If exudate is not observed, insert the swab inot the endocervical canal and rotate the swab.

3.  Remove from vagina and place into the Eswab transport tube and bend the swab shaft at the breakpoint indicated by the line marked on the swab.  Bend the shaft AWAY from you.

4.  Discard the broken handle of the swab, replace the cap on the tube.

VAGINAL:

1.  Using the BD Eswab, insert into the vagina.  Collect discharge or vagila secretions form the mucosa high in the vaginal canal.

2.  Remove from vagina and place into the Eswab transport tube and bend the swab shaft at the breakpoint indicated by the line marked on the swab.  Bend the shaft AWAY from you.

3.  Discard the broken handle of the swab, replace the cap on the tube.

URETHRAL:

**COLLECT SPECIMEN 1-2 HOURS OR MORE AFTER URINATION**

1.  Insert the Eswab mini-tip (green top) swab 2-4 cm into the endourethra and gently rotate.  Leave in place for 1-2 seconds and withdraw it.

2.  Remove from urethra and place into the Eswab transport tube and bend the swab shaft at the breakpoint indicated by the line marked on the swab.  Bend the shaft AWAY from you.

3.  Discard the broken handle of the swab, replace the cap on the tube.

THROAT:

1.  Depress the tongue gently with a tongue depressor. 

2.  Using the Eswab, swab surfaces between the tonsillar pillars and behind the uvula.  Avoid touching any othe surfaces of the mouth.

3.  Sweep the swab back and forth across the posterior pharnyx, tonsillar areas, and any inflamed or ulcerated areas to obtain the sample.

4.  Remove from the mouth and place into the Eswab transport tube and bend the swab shaft at the breakpoint indicated by the line marked on the swab.  Bend the shaft AWAY from you.

5.  Discard the broken handle of the swab, replace the cap on the tube.

RECTAL:

1.  Pass the tip of the Eswab approximately 2cm beyond the anal sphincter.  Carefully rotate the swab to sample the anal crypts and withdraw it.

2.  Remove from the anus and place into the Eswab transport tube and bend the swab shaft at the breakpoint indicated by the line marked on the swab.  Bend the shaft AWAY from you.

3.  Discard the broken handle of the swab, replace the cap on the tube.

 

** Label the Eswab with patient name (first and last) or other unique identifier, patient hospital
identification number, date and time of collection, collector initials, specimen source, and test(s) being ordered.  

**Promptly forward specimen to laboratory at ambient temperature.

Specimen Minimum Volume

One swab

Specimen Stability Information

Specimen Type Temperature Time
Eswab Ambient Within 24 hours

Reference Values

An interpretive report will be provided

Cautions

False negative results may occur if delay in transport to the laboratory or an inappropriate transport

 

For testing of sources other than what is listed, please refer to alternative testing methods such as nucleic acid amplification assays

 

Urine is not acceptable for this test

Day(s) Performed

Sunday through Saturday

Report Available

Preliminary report available within 24 hours.  Final report available at 48 hours.