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HelpTEST ID FFES Estradiol Free, Serum (includes Estradiol and SHBG)
Reporting Name
Free Estradiol with SHBGSpecimen Type
Serum RedSpecimen Required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel tube
Submission Container/tube: Plastic vial
Specimen Volume: 3 mL
Collection Instructions:
1. Within 45 minutes of collection, centrifuge and aliquot 3 mL of serum into a plastic vial.
2. Freeze immediately after separation and send frozen.
Specimen Minimum Volume
1 mL (Note: This volume does not allow for repeat testing.)
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Frozen (preferred) | 180 days |
| Ambient | 48 hours | |
| Refrigerated | 48 hours |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS); Equilibrium Dialysis; Immunoradiometric Assay (IRMA)
Reject Due To
| Plasma | Reject |
Reference Values
Estradiol, Serum MS
Units: pg/mL
Newborn:
Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15.
Males <6 m:
Levels increase to 10 - 32 between 30 and 60 days, then decline to prepubertal levels of <15 by six months.
Females <1 y:
Levels increase to 5.0 - 50 between 30 and 60 days, then decline to prepubertal levels of <15 during the first year.
Prepubertal: <15
Adult Males: 8.0 - 35
Adult Females:
Follicular: 30 - 100
Luteal: 70 - 300
Postmenopausal: <15
Free Estradiol, Percent
Units: %
Adult Males: 1.7 - 5.4
Adult Females: 1.6 - 3.6
Free Estradiol, Serum
Units: pg/mL
Adult Males: 0.2 - 1.5
Adult Females: 0.6 - 7.1
Sex Hormone Binding Globulin
Units: nmol/L
Infants (1 - 23m): 60.0 - 252.0
Prepubertal: 72.0 - 220.0
Pubertal:
Males:16.0 - 100.0
Females: 36.0 - 125.0
Adult Males:
20 - 49 y: 16.5 - 55.9
>49y: 19.3 - 76.4
Adult Females:
20 - 49y: 24.6 - 122.0
>49y: 17.3 - 125.0
Day(s) Performed
Varies
Report Available
10 to 16 daysPerforming Laboratory
Esoterix EndocrinologyCPT Code Information
82681 – Free Estradiol
84270 - Sex Hormone binding globulin
82670 - Estradiol