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TEST ID FFES Estradiol Free, Serum (includes Estradiol and SHBG)

Reporting Name

Free Estradiol with SHBG

Specimen Type

Serum Red


Specimen Required


Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel tube

Submission Container/tube: Plastic vial

Specimen Volume: 3 mL Serum

Collection Instructions:

1. Within 45 minutes of collection, centrifuge and aliquot 3 mL of serum into a plastic vial.

2. Freeze immediately after separation and send frozen.

Additional Information: To avoid delays when requesting multiple tests on frozen specimens, submit separate specimens for each test requested.


Specimen Minimum Volume

Serum: 1 mL; Note: This volume does not allow for repeat testing.

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen (preferred) 180 days
  Ambient  48 hours
  Refrigerated  48 hours

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS); Equilibrium Dialysis; Immunoradiometric Assay (IRMA)

Reject Due To

Plasma Reject

Reference Values

Estradiol, Serum MS

Newborn: Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15 pg/mL.

Males <6 months: Levels increase to 10-32 pg/mL between 30 and 60 days, then decline to prepubertal levels of <15 pg/mL by 6 months.

Females <1 year: Levels increase to 5.0-50 pg/mL between 30 and 60 days, then decline to prepubertal levels of <15 pg/mL during the first year.

Prepubertal: <15 pg/mL

 

Adult Males: 8.0-35 pg/mL

Adult Females:

Follicular: 30-100 pg/mL

Luteal: 70-300 pg/mL

Postmenopausal: <15 pg/mL

 

Free Estradiol, Percent

Adult Males: 1.7-5.4 %

Adult Females: 1.6-3.6 %

 

Free Estradiol, Serum

Adult Males: 0.2-1.5 pg/mL

Adult Females: 0.6-7.1 pg/mL

 

Sex Hormone Binding Globulin

Infants (1 month-23 months): 60.0-252.0 nmol/L

Prepubertal: 72.0-220.0 nmol/L

 

Pubertal:

Males:16.0-100.0 nmol/L

Females: 36.0-125.0 nmol/L

 

Adult Males:

20 years-49 years: 16.5-55.9 nmol/L

>49 years: 19.3-76.4 nmol/L

 

Adult Females:

20 years-49 years: 24.6-122.0 nmol/L

>49 years: 17.3-125.0 nmol/L

Day(s) Performed

Varies

Report Available

10 to 16 days

Performing Laboratory

Esoterix Endocrinology

CPT Code Information

82681-Free Estradiol

84270-Sex Hormone binding globulin

82670-Estradiol