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TEST ID CYCL Cyclospora Stain, Feces

Reporting Name

Cyclospora Stain

Specimen Type

Fecal


Ordering Guidance


Cryptosporidium oocysts do not reliably stain with the modified safranin stain. If Cryptosporidium is suspected, the specific enzyme-linked immunosorbent assay for this organism is recommended; order CRYPS / Cryptosporidium Antigen, Feces. To detect this organism as well as other common infectious causes of diarrhea, order GIP / Gastrointestinal Pathogen Panel, PCR, Feces.



Specimen Required


Patient Preparation: Patient should avoid use of antidiarrheal medication (eg, loperamide [Imodium-AD] or Pepto-Bismol).

The presence of barium will interfere with this test.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Preserved stool

Supplies:

-ECOFIX Stool Transport Vial (Kit) (T219)

-Formalin-Meridian 10% Buffered Neutral (T466)

Container/Tube:

Preferred: ECOFIX Stool Transport Vial (Kit)

Acceptable: 10% Buffered Formalin Stool Transport (Kit), Sodium Acetate Formalin (SAF)

Specimen Volume: 10 g

Specimen Stability Information: Ambient 21 days (preferred)/Refrigerated 21 days

 

Acceptable

Specimen Type: Unpreserved stool

Supplies: Stool container, Small (Random), 4 oz (T288)

Container/Tube: Stool container

Specimen Volume: 5 g

Specimen Stability Information: Refrigerated 3 days (preferred)


Specimen Minimum Volume

Preserved stool: 1 g
Unpreserved stool: 2 g

Specimen Stability Information

Specimen Type Temperature Time
Fecal Varies

Testing Algorithm

For other diagnostic tests that may be of value in evaluating patients with diarrhea; the following are available:

-Laboratory Testing for Infectious Causes of Diarrhea

-Parasitic Investigation of Stool Specimens Algorithm

Method Name

Safranin Stain

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values

Negative

If positive, reported as Cyclospora cayetanensis detected.

Day(s) Performed

Monday through Saturday

Report Available

2 to 4 days

Specimen Retention Time

3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

CPT Code Information

87015

87207

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Microbiology Test Request (T244)

-Gastroenterology and Hepatology Test Request (T728)

Useful For

Identifying Cyclospora cayetanensis as a cause of infectious gastroenteritis