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TEST ID CRYGIAAG CRYPTOSPORIDIUM & GIARDIA ANTIGEN, STOOL

Important Note

Rapid test for the qualitative, simultaneous detection of Cryptosporidium oocyst and Giardia cyst antigens in human stool specimens

Giardia and Cryptosporidium are recognized as two of the most frequent causes of parasitic intestinal disease.  Transmission is usually through ingestion of contaminated food or water.

Infectious

Performing Laboratory

NRLS-Microbiology

Specimen Type

Stool

Specimen Required

Stool collection:

Pass stool into a clean, dry container. 

--The use of a bed pan or a large plastic bag into a waste basket can be used to catch the specimen

--Collect stool from diaper lined with plastic. If infant or child has diarrhea, line the diaper with plastic wrap or use a pediatric urine bag placed over the rectum to collect a specimen that is not contaminated with urine.

CAUTION:  Never leave plastic wrap against a child's skin for more than 30 minutes at a time.

 

-Label cup with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature.

 

For Cary Blair media (Orange top transport) and/or the EcoFix transport (green top): 

1)  Open the lid of the tube containing the liquid. 

2)  Using the built-in spoon, place small spoonfuls of stool (sample the bloody, slimy, or watery portions) into the tube until the liquid level rises to the red line.   

3)  If the stool is formed, try to sample small amounts from each end/side and the middle. 

4)  Mix the contents with the spoon, then twist the cap tightly closed and shake to mix well.

For the FecalSwab transport:

1) Remove the swab from the package, making sure to only hand the swab by the shaft

2)  Collect the stool onto the swab by inserting the swab tip into the stool sample and rotate.  Be sure to sample the blood, slimy, or watery areas.  DO NOT try to collect a large amount of sample on the swab, but fecal material must be visible on the swab.

3)  Carefully insert the swab into the vial of fluid that came with the swab (orange top tube) and mix the specimen into the fluid by swirling against the inside of the tube.

4)  Break off the swab at the perforation into the tube by holding the top of the swab shaft and bending toward you.  Discard the top portion of the swab and replace the orange lid.

5)  Shake well until mixed.  Confirm that the liquid level is not above the noted line on the tube.

 

-Label cup with patient name (first and last) or other unique identifier, patient hospital
identification number,  date and time of collection, collector initials, and test(s) being
ordered.
-Promptly forward specimen to laboratory at ambient temperature.

Specimen Minimum Volume

1.0 g

Specimen Stability Information

Specimen Type

Temperature

Time

Stool, preserved (10% formalin, EcoFix, Cary Blair, FecalSwab) Ambient (preferred) 72 hours
Stool, unpreserved

Refrigerated   

(2-8C)

72 hours
Stool, frozen

Frozen

(≤-10C)

90 days

Reference Values

An interpretive report will be provided

Day(s) Performed

Sunday through Saturday

Report Available

Within 24 hours

Methodology

Enzyme Immunoassay

 

Rejection Criteria

-Stool received in the laboratory outside of the stability requirements

-Stool received in expired transport media

-Stool contaminated with urine or toilet water

-Stool in a diaper

-Stool in PVA fixative