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HelpTEST ID BIOTN Biotin, Serum
Reporting Name
Biotin, SSpecimen Type
SerumOrdering Guidance
If testing for biotinidase deficiency is requested, order BIOTS / Biotinidase, Serum.
Shipping Instructions
Freeze specimens within 3 days of collection and send frozen.
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 30 days | |
| Refrigerated | 7 days | ||
| Ambient | 24 hours |
Method Name
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reject Due To
| Gross hemolysis | OK |
| Gross lipemia | OK |
| Gross icterus | OK |
Reference Values
≥18 years: ≤0.3 ng/mL
Reference values have not been established for patients who are <18 years of age.
Day(s) Performed
Tuesday
Report Available
2 to 7 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84591
Useful For
Measurement of biotin in serum
Assessment of biotin concentrations in individuals taking biotin supplements
Investigation of unexpected results from immunoassays that utilize biotin-streptavidin detection methods
This test is not useful as a screen for biotinidase deficiency.
Highlights
Serum biotin is usually at relatively low endogenous concentrations but can be found at high concentrations if vitamin and herbal supplements containing biotin are taken.
This test measures free biotin concentrations in serum and can be used to determine if a patient's high biotin concentrations are likely from biotin supplementation or treatment.