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HelpTEST ID ALKP Alkaline Phosphatase, Total and Isoenzymes, Serum
Necessary Information
Patient's age and sex are required.
Specimen Required
Patient Preparation: Fasting (8 hours) required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Within 2 hours of collection, centrifuge the specimen.
2. For red top tubes, immediately aliquot into a plastic vial.
3. For serum gel tubes, serum may sit on gel refrigerated but must be aliquoted within 7 days.
Useful For
Aid in the diagnosis and treatment of liver, bone, intestinal, and parathyroid diseases
Determining the tissue source of increased alkaline phosphatase (ALP) activity in serum
Differentiating between liver and bone sources of elevated ALP
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ALP | Alkaline Phosphatase, S | Yes | Yes |
| ALPI | Alkaline Phosphatase Isoenzymes, S | No | Yes |
Method Name
ALP: Colorimetric
ALPI: Electrophoresis
Reporting Name
Alkaline Phosphatase, Tot and Iso,SSpecimen Type
SerumSpecimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Ambient | 7 days | ||
| Refrigerated | 7 days |
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
| Gross icterus | Reject |
Reference Values
ALKALINE PHOSPHATASE
Males
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 116-468 U/L
15-<17 years: 82-331 U/L
17-<19 years: 55-149 U/L
≥19 years: 40-129 U/L
Females
0-14 days: 83-248 U/L
15 days-<1 year: 122-469 U/L
1-<10 years: 142-335 U/L
10-<13 years: 129-417 U/L
13-<15 years: 57-254 U/L
15-<17 years: 50-117 U/L
≥17 years: 35-104 U/L
ALKALINE PHOSPHATASE ISOENZYMES
Ages:
≤17 years: Refence values have not been established for patients less than 18 years of age.
≥18 years:
Liver %: 30.2-74.7
Liver U/L: 15.8-71.9
Bone %: 23.8-68.3
Bone U/L: 12.0-56.7
Intestine %: ≤22.5
Intestine U/L: ≤12.6
Day(s) Performed
Tuesday through Saturday
Report Available
3 to 5 daysSpecimen Retention Time
7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84075
84080