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HelpTEST ID 23BPT 2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, 24 Hour, Urine
Reporting Name
2,3-dinor 11B-Prostaglandin F2a, USpecimen Type
UrineOrdering Guidance
A 24-hour urine collection is the preferred specimen type, but a random specimen is also acceptable for 2,3-dinor 11beta-prostaglandin F2 alpha; order 23BPR / 2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, Random, Urine.
If the total volume provided is less than 300 mL or the specimen does not meet the 24-hour urine requirements, this test will be canceled and 23BPR ordered and performed.
Necessary Information
Specimen volume (in milliliters) and duration (in hours) are required.
Specimen Required
Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAIDs) may have decreased concentrations of prostaglandin F2 alpha. If medically feasible, for 2 weeks before specimen collection, patient should not take aspirin and for 72 hours before specimen collection, patient should not take NSAIDs.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Container/Tube: Plastic, 5-mL tube
Specimen Volume: 5 mL
Collection Instructions:
1. Collect urine refrigerate for 24 hours.
2. No preservative preferred.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Frozen | 30 days | |
| Ambient | 8 hours |
Special Instructions
Method Name
T23BP: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
CRT24: Enzymatic Colorimetric Assay
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Reference Values
<1802 pg/mg creatinine
Day(s) Performed
Tuesday, Thursday
Report Available
3 to 8 daysSpecimen Retention Time
14 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
CPT Code Information
84150
Useful For
Screening for mast cell activation disorders including systemic mastocytosis using 24-hour urine specimens
Highlights
2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is elevated in the urine of patients with systemic mastocytosis (SM).
This test should be used as a screening test for SM.
When 2,3 BPG is used in combination with urinary leukotriene E4 and N-methylhistamine, the sensitivity for SM detection increases to 90%.
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| T23BP | 2,3-dinor 11B-Prostaglandin F2a | No | Yes |
| CRT24 | Creatinine, 24 HR, U | No | Yes |
Clinical Information
2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is the most abundant metabolic product of prostaglandins released by activated mast cells. Systemic mastocytosis (SM) is a disease in which clonally derived mast cells accumulate in peripheral tissues. Degranulation of these mast cells releases large amounts of histamines, prostaglandins, leukotrienes, and tryptase.
The World Health Organization diagnostic criteria for SM require the presence of elevated mast cell counts on a bone marrow biopsy and one of the following minor criteria:
-Abnormal mast cell morphology
-KIT Asp816Val variant
-CD25-positive mast cells
-Serum tryptase greater than 20 ng/mL
Alternatively, SM diagnosis can be made with the presence of 3 minor criteria in the absence of abnormal bone marrow studies.
Measurement of mast cell mediators in blood or urine is less invasive and is advised for the initial evaluation of suspected cases. Elevated levels of serum tryptase, urinary N-methylhistamine, 2,3 BPG, or leukotriene E4 are consistent with the diagnosis of systemic mast cell disease.